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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 414
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Cell_line_name |
647-V
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Brief_description |
confirmed as human with IEF of AST, NP established from a male patient with a
primary urothelial bladder carinoma (malignant, grade 2)
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Description |
human urothelial bladder carcinoma established from a male patient with a primary
urothelial bladder carinoma (malignant, grade 2) confirmed as human with IEF of AST,
NP
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Depositor |
Dr. W. A. Schulz, Urologische Klinik, Duesseldorf, Germany
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Morphology |
adherent epithelial-like cells growing in monolayers
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Culture_conditions |
85% Dulbecco's MEM + 15% FBS + 2 mM L-glutamine split confluent culture 1:5 to
1:6 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml
medium at 37C with 5-10% CO2 cell harvest of ca. 10-15 x 106 cells/175 cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -,
SMRV -
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Properties |
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +,
desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.0 x 106 cells/ampoule
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Doubling_time |
ca. 30 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-
60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32),
del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24),
del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p)
associated with bladder cancer
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Bibliographic_reference |
Elliott et al., Cancer Res, 36: 37 (1976) [PMID: 944089
] |
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